PRE-CONFERENCE FOCUS DAY
The Exosome Characterization & Analytical Development Focus Day will deep dive into key insights and interactive discussions to support your exosomes and EVs production, isolation, and identification using the most established and robust techniques, using the most efficient tools, and ensuring high quality to advance progress from research to clinic effectively.
8:00 am Check-In & Morning Coffee
8:50 am Chair’s Opening Remarks
Establishing Robust Analytical Methods to Better Characterize Exosomes with Improved Quantification & Precision
9:00 am Case Study: Utilizing MSC-derived Extra-Cellular Vesicles to Repair Infected Cells
Synopsis
- How to effectively purify MSC-derived extra-cellular vesicles
- Effectively characterizing cellular repair strategies
- Presenting in vivo and in vitro assays used for cellular repair
9:30 am Leveraging Mechanical Regulation of Cell Phenotype for Scalable EV Production
Synopsis
- How to design a mechanical environment for cell culture that’s compatible with perfusion and/or flow-based bioreactor cultures for large-scale EV production
- Assessing effects of mechanical cell culture conditions on EV contents and activity
- Evaluating versatility of mechanical control of EV production and activity across multiple producer cell types
10:00 am Cracking the Code: Predicting Exosome Pharmacology Through Physicochemical Insights
Synopsis
- The critical need for predictive physicochemical testing in exosome drug development
- Key challenges in interpreting and correlating physicochemical data with pharmacological outcomes
- Our breakthrough case study: A successful prediction-to-performance story
NEW DATA!
10:30 am Morning Break & Networking
Improving Purification Methods to Ensure Consistent & Homogonous Exosome Isolation for Therapeutic Use
11:00 am High Purity & Throughput Isolation of Exosomes via Capillary-Channeled Fiber Chromatography
Synopsis
- The efficient isolation of high-purity populations of EVs is a limitation in fundamental biochemistry, clinical diagnostics, and vector production
- Hydrophobic interaction chromatography (HIC) on capillary-channeled polymer phases provides high purity and throughput with low capital costs
- Examples of isolation characteristics will be presented for matrices ranging from urine to plasma, cell cultures, and bovine milk
11:30 am Strengthening & Expanding siRNA Drug Capability via EV Delivery System
Synopsis
- Building a leading chromatographic exosome purification capability for suspension and adherent cell cultures
- Developing a proprietary payload loading technologies for small interfering RNAs and active protein drugs.
- Therapeutically significant targets can be efficiently regulated in lung, skin and solid tumor disease models, paving the way for further clinical development
12:00 pm Panel Discussion: Harnessing Novel Technologies to Effectively Measure, Isolate & Characterise Exosome Cargoes
Synopsis
- What novel technologies are coming onto the market to facilitate exosome characterization and advance the development of future therapeutics?
- What are the current challenges of isolating, purifying, and measuring exosomes effectively?
- How to effectively leverage novel imaging tools to successfully analyze exosome morphology and modifications?
1:00 pm Lunch Break & Networking
Demonstrating Effective Exosome Analytical & Quality Control Standards to Ensure Consistency & Reproducibility for Clinical Progress
2:00 pm Membrane Characterization of EVs from Genetic-Engineered Human Thymic Epithelial Cells: Implications in T Lymphopoiesis
Synopsis
- Generate and characterize genetic-engineered human thymic epithelial cells expressing the Notch ligand DLL4 and the derived EVs/exosomes
- Analysis of EVs by multi-color flow cytometry and cluster analysis of EV heterogeneity
- Characterization of EV ligand-receptor interaction with hematopoietic stem cells (HSCs)
2:30 pm Roundtable Discussion: Utilizing the Most Optimal Analytical Assays to Support Your Exosome Drug Product Release Strategies
Synopsis
- Evaluating conventional and novel instruments to analyze exosomes across ELISA, acoustic Imaging, flow cytometry, and more to confidently select your technology
- Ensuring analytical methods are validating, qualifying, and reproducible to ensure safety and sterility of the exosome product
- One vs multiple potency assays – How to address complex mechanisms of action within the production phase